The Influence of Cytochrome C on the Polycondensation of Silicic Acid

The polycondensation kinetics of silicic acid was measured cryoscopically in a temperature interval between 5 ◦C and 35 ◦C. Unlike polyamines such as polyallylamine, polylysine or polyarginine, catalitical amounts of Cytochrom c have a distinct retarding influence on the polycondensation kinetics. The Arrhenius analysis of the experimental reaction rates with and without Cytochrome c determines an invariant activation energy of E A = (69.9±0.7) kJ/mol, while the pre-exponential factors differ by ∆κ0 = (1×108±8×107) s−1. The latter can be attributed to a three-times pejoration of a sterical factor, which then indicates, that the adsorption of reactive silicic acid oligomers on the protein is dominating the reaction rate.